BBB is a highly selective semipermeable border formed by microvascular endothelial cells found in the brain, which communicate with other CNS cells (astrocytes and pericytes) and respond accordingly to pathological environments to regulate cell function and disease development.
The BBB is responsible for maintaining central nervous system homeostasis by preventing toxic or harmful molecules from entering the CNS, transporting nutrients to the brain, and eliminating metabolites. There are several disorders related to the brain, such as Neurological-HIV, strokes, brain tumours, and other neurodegenerative diseases which cause the BBB to be more permeable. In contrast, the semipermeable BBB restricts the movement of larger molecules, including drugs and proteins (>500 kDa), which contributes to minimal drug absorption into the CNS. The most challenging aspect of developing therapeutics for CNS neurodegenerative disorders lies in this issue. While the dynamic and adaptable characteristics of BBB influence the delivery of drugs to the CNS and promote disease progression, a comprehensive understanding of the composition and functions of BBB provide a platform for the development of novel innovative approaches to drug delivery to the CNS. Numerous in vitro models of the BBB have been developed and advanced because of methodical and scientific interest in the BBB’s physiology and pathology.
Physiological relevance of an in vitro BBB model depends not only on the cells expressing BBB markers, but also on their ability to form the right junctions, ensuring barrier integrity.
DA-TA Biotech provides clients with a three-dimensional blood-brain barrier model by cultivating brain endothelial cells and stratified pericytes and astrocytes. The model improves tight junction formation and polarization of endothelial cells. Improved development of endothelial cell contacts is essential. The development of these conditions mimics the in vitro model of BBB. Using these models, drug screening campaigns can be supported, as well as questions about the mechanisms of neurodegenerative diseases. Compounds can be studied both for their transport across the barrier as well as their effect on the structure and function of the BBB using this service.
In addition we can also provide in vivo digestion models in collaboration with BiotechFarm.